Research Training Group 2498

Research Training Group 2498

Communication and Dynamics of Plant Cell Compartments

Projects


Stress-induced redistribution of lipids between plastidial and extraplastidial membranes

Project Leader// Prof. Dr. Ingo Heilmann

Graduate// Monique Matzner

Martin-Luther-University Halle-Wittenberg
Institute of Biochemistry and Biotechnology
Plant Biochemistry


Plants adapt their physiology and growth to match their environment. Salt and osmotic stress are important environmental cues and result in changes in gene expression within minutes to hours. Physiologically, hyperosmotic stress results in an immediate (i.e., within few seconds) loss of turgor, accompanied by plasmolysis of the plasma membrane (within seconds to minutes) and followed by the internalization of membrane area by bulk- flow endocytosis. Despite their importance, the mechanisms ensuring the integrity of the plant plasma membrane under osmotic stress are not well studied. The degree of unsaturation of membrane phospholipids is one critical factor stabilizing the plasma membrane during hyperosmotic stress. We have previously reported that hyperosmotic stress induces increased incorporation of long- chain polyunsaturated fatty acids (LCPUFAs) into membrane lipids. Interestingly, the content of LCPUFAs decreases in plastidial galactolipids, such as monogalactosyldiacylglycerol (MGDG), concomitant with the appearance of LCPUFAs in plasma membrane phospholipids. These findings led us to hypothesize that LCPUFA-containing plastidial lipids, such as MGDG, are sources for LCPUFAs that can be mobilized in a stress- dependent manner to be incorporated into extraplastidial membrane lipids. So far it is unclear how stress-induced mobilization of LCPUFAs from plastids to extraplastidial membranes may be achieved, and whether there is reimport of LCPUFAs or of lysolipids into plastids at later stages of adaptation to the stress. It is the overall goal of this project to elucidate the mode(s) of stress-induced LCPUFA trafficking from and to the plastid and its relation to plant membrane trafficking. The project is subdivided in the following work packages:
Unchanged: Plants adapt their physiology and growth to match their environment. Salt and osmotic stress are important environmental cues and result in changes in gene expression within minutes to hours. Physiologically, hyperosmotic stress results in an immediate (i.e., within few seconds) loss of turgor, accompanied by plasmolysis of the plasma membrane (within seconds to minutes) and followed by the internalization of membrane area by bulk- flow endocytosis. Despite their importance, the mechanisms ensuring the integrity of the plant plasma membrane under osmotic stress are not well studied. The degree of unsaturation of membrane phospholipids is one critical factor stabilizing the plasma membrane during hyperosmotic stress. We have previously reported that hyperosmotic stress induces increased incorporation of long- chain polyunsaturated fatty acids (LCPUFAs) into membrane lipids. Interestingly, the content of LCPUFAs decreases in plastidial galactolipids, such as monogalactosyldiacylglycerol (MGDG), concomitant with the appearance of LCPUFAs in plasma membrane phospholipids. These findings led us to hypothesize that LCPUFA-containing plastidial lipids, such as MGDG, are sources for LCPUFAs that can be mobilized in a stress- dependent manner to be incorporated into extraplastidial membrane lipids. So far it is unclear how stress-induced mobilization of LCPUFAs from plastids to extraplastidial membranes may be achieved, and whether there is reimport of LCPUFAs or of lysolipids into plastids at later stages of adaptation to the stress. It is the overall goal of this project to elucidate the mode(s) of stress-induced LCPUFA trafficking from and to the plastid and its relation to plant membrane trafficking. The project is subdivided in the following work packages:

  1. Monitor organellar contacts during osmotic stress by confocal microscopy
  2. Document stress-induced lipid-redistribution by time-resolved lipid analysis
  3. Test LCPUFA-containing lipid-redistribution in relevant Arabidopsis mutants
  4. Correlate osmotolerance and LCPUFA content of plasma membrane lipids
  5. Monitor plastid function during osmotic- stress